In Silico Identification of Microbial Partners to Form Consortia with Anaerobic Fungi
نویسندگان
چکیده
Lignocellulose is an abundant and renewable resource that holds great promise for sustainable bioprocessing. However, unpretreated lignocellulose is recalcitrant to direct utilization by most microbes. Current methods to overcome this barrier include expensive pretreatment steps to liberate cellulose and hemicellulose from lignin. Anaerobic gut fungi possess complex cellulolytic machinery specifically evolved to decompose crude lignocellulose, but they are not yet genetically tractable and have not been employed in industrial bioprocesses. Here, we aim to exploit the biomass-degrading abilities of anaerobic fungi by pairing them with another organism that can convert the fermentable sugars generated from hydrolysis into bioproducts. By combining experiments measuring the amount of excess fermentable sugars released by the fungal enzymes acting on crude lignocellulose, and a novel dynamic flux balance analysis algorithm, we screened potential consortia partners by qualitative suitability. Microbial growth simulations reveal that the fungus Anaeromyces robustus is most suited to pair with either the bacterium Clostridia ljungdahlii or the methanogen Methanosarcina barkeri—both organisms also found in the rumen microbiome. By capitalizing on simulations to screen six alternative organisms, valuable experimental time is saved towards identifying stable consortium members. This approach is also readily generalizable to larger systems and allows one to rationally select partner microbes for formation of stable consortia with non-model microbes like anaerobic fungi.
منابع مشابه
Supplementary Materials: In Silico Identification of Microbial Partners to Form Consortia with Anaerobic Fungi
In this section we provide more details regarding the dFBA formulation we used. The primary difference between our algorithm and that of [1] is that we impose only a single secondary LP on the system. We assume only that the flux derivative change between time steps is small. In [1], two additional assumptions per integrated variable need to be imposed on the system i.e. the sense of each LP (m...
متن کاملInvestigation of Dust Microbial Community and Identification of its Dominance Species in Northern Regions of Sistan and Baluchestan Province
Many studies have been done on various properties of dust and one of the most important characteristics of dust is the ability to carry different microorganisms from the source points. The aim of this study was to investigate the bacterial and fungal community of dust and to identify its dominance species in a single event of intense dust storm, in the northern regions of Sistan and Blauchestan...
متن کاملEffect of anaerobic HRT on biological phosphorus removal and the enrichment of phosphorus accumulating organisms.
The purpose of this research was to develop a better understanding of the dynamic effects of anaerobic hydraulic retention time (HRT) on both enhanced biological phosphorus removal (EBPR) performance and enrichment of phosphorus accumulating organisms (PAOs). The research was conducted using laboratory-scale sequencing batch reactors inoculated with mixed microbial consortia and fed real wastew...
متن کاملSeasonal variations of microbial community in a full scale oil field produced water treatment plant
This study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. The community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and Illumina high-throughput sequencing, respectively. Chemical oxygen demand eff...
متن کاملApplication of tetranucleotide frequencies for the assignment of genomic fragments.
A basic problem of the metagenomic approach in microbial ecology is the assignment of genomic fragments to a certain species or taxonomic group, when suitable marker genes are absent. Currently, the (G + C)-content together with phylogenetic information and codon adaptation for functional genes is mostly used to assess the relationship of different fragments. These methods, however, can produce...
متن کامل